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| Lima,Marcos Antonio Barbosa de; Nascimento,Aline Elesbão do; Souza,Wanderley de; Fukushima,Kazutaka; Campos-Takaki,Galba Maria de. |
| The content of inorganic polyphosphate and the polymeric degree of these compounds were evaluated during the growth of Cunninghamella elegans in medium containing varying orthophosphate (Pi) concentrations. For this purpose, a combination of chemical methods for polyphosphate extraction and ultrastructural cytochemistry were used. The orthophosphate and glucose consumption was also determined during the fungal cultivation. At Pi concentrations of 0.5, 2.5 and 0.0 g/L, the maximum amounts of biomass were 3.18, 3.29 and 0.24 g/L, respectively. During growth the cells accumulated Pi from the medium. At three days of growth the biomass consumed up to 100 and 95% of Pi from the media at initial concentrations of 0.5 and 2.5 g/L, respectively. Polyphosphate was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polyphosphate; Accumulation; Ultrastructural cytochemistry; Cunninghamella elegans; Ortophosfate. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400016 |
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| Shari'a,Allana Elesbão do Nascimento; Nascimento,Aline Elesbão do; Lima,Marcos Antonio Barbosa de; Campos-Takaki,Galba Maria de; Souza,Wanderley de. |
| The inorganic phosphorus content, and distribution, structure and localization of polyphosphate in mycelia of zygomycetous fungi was evaluated. Ultrastructural cytochemistry was successfully used to identifying the localization and distribution of polyphosphate in Absidia cylindrospora, Gongronella butleri and Mucor javanicus. The results revealed differences in the cytochemical staining pattern in all species studied and a uniform labeling on the cellular surface (cell wall and citoplasmic membrane). Reaction products were observed in intracellular structures and cellular membrane. Intracellular staining was observed in trabecullar, vacuolar and vesicular structures, in dense bodies and in the cytoplasm. However, the cytochemical staining intensity varied... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ultrastructural cytochemistry; Polyphosphate; Zygomycetes. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200004 |
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| Souza,Wanderley de. |
| Secretory processes play an important role on the biology and life cycles of parasitic protozoa. This review focus on basic aspects, from a cell biology perspective, of the secretion of (a) micronemes, rhoptries and dense granules in members of the Apicomplexa group, where these organelles are involved in the process of protozoan penetration into the host cell, survival within the parasitophorous vacuole and subsequent egress from the host cell, (b) the Maurer's cleft in Plasmodium, a structure involved in the secretion of proteins synthesized by the intravacuolar parasite and transported through vesicles to the erythrocyte surface, (c) the secretion of macromolecules into the flagellar pocket of trypanosomatids, and (d) the secretion of proteins which... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Parasitic protozoa; Cell secretion; Apicomplexa; Trypanosomatids; Encystation vesicles. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000200008 |
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